Fiber-coupled microscope for imaging neural activity
Our lab has developed a light-weight fiber-coupled head-mounted multiphoton microscope for imaging brain activity in awake behaving mice. In collaboration with Profs. Juliet Gopinath and Victor Bright, we employ electrowetting adaptive optics to allow for high-speed variable focus with no moving parts in a compact design.
Super resolution microscopy
We are developing a Stimulated Emission Depletion (STED) microscope for video-rate imaging of live cells at resolutions of ~ 40-50 nm. In collaboration with Professor Diego Restrepo, we are using STED to study calcium microdomains in cilia in olfactory sensory neurons, important for signal transduction in olfaction. Additional studies that can be performed using STED include studies dendritic spines, involved in learning, myelin, important for problem solving, motor coordination and sensory input, synapses, key for transmission of the neural signal and primary cilia, important in neuronal differentiation.